Pacific Biosciences of California, a genetic sequencing company, said it has made advancements in sequencing for the maize genome that may have implications for other complex crop and livestock genomes. The company said its single molecule, real-time sequencing and genome assembly was produced by scientists at Cold Spring Harbor Laboratory. The company said it is a resource for crop breeding and selection programs and serves as a model for how the company’s sequencing can contribute to affordably improving other economically valuable crops.
The assembly significantly reduced gaps in the existing reference genome with a more than 50-fold increase in contiguity compared to the previous assembly, the company said. The scientists also corrected mistakes in previous assemblies, added data to difficult regions such as centromeres and regulatory sequences, and provided new gene annotations using PacBio’s Iso-Seq method.